Plus reactions worked differently: they used T4 DNA polymerase, an enzyme with strong 3’ to 5’ exonuclease activity, meaning it can chew back the end of a DNA strand. In the presence of only one dNTP, T4 DNA polymerase would degrade each fragment from its 3’ end until it reached a nucleotide complementary to that dNTP, at which point the exonuclease activity would be inhibited. This ensured that all fragments in a given plus reaction ended with the same nucleotide.
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Ранее гастроэнтеролог Саураб Сети рассказал, какие продукты незаметно отравляют организм. Клубника, утверждает он, может привести к гормональному дисбалансу.
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